sampledeep sleep
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This product is sold strictly for in lab conditions (in vitro) research purposes. Not approved for human consumption.
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DSIP
Delta Sleep-Inducing Peptide | Sleep & Stress Modulator
In short
Studied for how it may help your body achieve a deeper, more restful sleep. Research suggests it could improve how well you relax and recover overnight.
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DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring peptide initially isolated for its ability to influence brain activity. At the molecular level, it modulates various neurotransmitter systems by interacting with GABA and NMDA receptors and regulating the hypothalamic-pituitary-adrenal axis. Scientists are studying it in laboratory and animal models to understand its potential role in regulating sleep, stress, and pain perception. This product is intended strictly for scientific research purposes and is not an approved medication or therapeutic agent for human use.
Research reagent for laboratory use only. Not a medicine. For medical questions, consult a physician.
Scientific review
Written by
Калина Тодорова
Magister Pharmaciae, MSc Pharmacy
Reviewed by
Борис Маринов
MSc Biochemistry & Molecular Biology
Reviewed on
DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nonapeptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu) discovered in 1974 in rabbit brain. Originally isolated for its sleep-promoting properties, DSIP has since been found to have diverse neuromodulatory effects including stress reduction, pain modulation, and endocrine regulation, though its exact mechanisms and clinical efficacy remain subjects of ongoing research.
EEG delta wave activation Reduced cortisol stress response Sleep architecture modulation No known dose-limiting tolerance
Modulates multiple neurotransmitter systems including GABA enhancement, NMDA receptor interaction, and endogenous opioid system modulation. Also regulates hypothalamic-pituitary-adrenal axis.
Promotes delta wave sleep, the most restorative sleep phase for physical recovery
Reduces sleep latency and nocturnal awakenings without morning grogginess
Supports natural sleep cycles rather than forcing sedation like traditional sleep aids
Dosing
The typical regimens you'll see in the published research - for reference when planning in vitro or in vivo experiments. Not medical advice.
Disclaimer
These are regimens discussed in the research literature, not medical advice. Consult a healthcare provider before use.
Timing
Evening administration 30-60 minutes before intended sleep
[Calculator]
Change water and dose · results update in real time
from product
Visual indicator
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10marks
Standard insulin syringe 1 ml (100 marks)
Concentration
2.50mg/ml
Dose volume
0.100ml
U-100 units
10units
Doses per vial
20doses
Recommended needle
29G–30G / 1 ml insulin
Small volume — draw slowly; 30G works for finer precision
Cycle planner: vials for 12 weeks, cost, schedule
Opens full calculator
Results are reference values - for in vitro work. Verify against the published literature for the specific peptide.
[Step by step]
6 steps from a sealed vial to the injection. Plain language.
Peptide vial (5 mg), bacteriostatic water vial, 1 larger syringe (3-5 ml) for drawing water and 1 insulin (1 ml) for doses. Alcohol swabs for sanitizing the caps.
Wash your hands before starting.
Wipe the rubber cap of the water vial with an alcohol swab. Insert the needle, invert the vial and draw exactly 2 ml. Get the exact volume from the calculator.
Larger syringe = smaller error in volume.
Wipe the peptide vial cap. Tilt the vial 45° and release the water SLOWLY down the inner wall. Peptides are fragile — a direct stream onto the powder denatures them.
SLOWLY · DOWN THE WALL · NOT ONTO THE POWDER.
Hold the vial between both palms and swirl slowly. DO NOT shake or flip sharply — peptides break down under mechanical stress.
DON'T SHAKE. Like tea — not like a cocktail.
The powder should fully dissolve. The solution is clear, no visible particles. If cloudy or with sediment — discard (wrong water or bad batch).
Clear = OK. Cloudy = discard.
With the insulin syringe draw the exact marks from the calculator. Swab the skin (abdomen 5 cm around the navel, thigh, or buttock), pinch a fold, insert at 45-90° and inject slowly.
Rotate sites every injection to avoid lipohypertrophy.
Schedule
Evening administration 30-60 minutes before intended sleep
Clean vial tops with alcohol swab and let dry completely
For 1mg vial, add 1ml BAC water (creates 1mg/ml or 1000mcg/ml)
Inject water slowly down vial side to prevent foaming
Gently roll between palms - do not shake
Solution should be clear and colorless
For 100mcg dose, draw 0.1ml (10 units on insulin syringe)
Store reconstituted peptide in refrigerator immediately
Generally well-tolerated with minimal side effects
Some users report mild drowsiness or dizziness initially
Occasional reports of headaches in sensitive individuals
No tolerance or dependence reported in studies
Avoid driving until effects are known
Not recommended during pregnancy or breastfeeding
References
Links to peer-reviewed publications on PubMed, cited in the peptide's scientific profile.
Stroke Recovery in Rats (2021)
Rats | 120 μg/kg nasal | 7 days | Enhanced motor recovery
Intranasal DSIP administration before and after induced stroke accelerated motor function recovery without significantly reducing infarct size, suggesting neuroprotective mechanisms.
Chronic Insomnia Double-Blind Study (1991)
Human | 15-30 nmol | 6 consecutive nights | Improved sleep efficiency
Double-blind crossover study showed DSIP improved objective sleep quality with higher sleep efficiency and shorter sleep latency compared to placebo, though subjective improvements were modest.
Endocrine Modulation Research (1988)
Human/Animal | Various doses | Multiple studies | Hormone regulation
Multiple studies demonstrated DSIP modulates ACTH, cortisol, and growth hormone release, suggesting broad neuroendocrine regulatory effects beyond sleep.
Chronic Pain Pilot Study (1984)
Human | 25 nmol IV | 10 days | 6/7 patients improved
DSIP significantly reduced pain levels in 6 of 7 patients with chronic pain conditions including migraine and tinnitus, with simultaneous reduction in accompanying depression.
Alcohol and Opioid Withdrawal (1984)
Human | Variable doses | 5-10 days | Symptom reduction
Clinical reports showed DSIP administration helped manage withdrawal symptoms in both alcohol and opioid-dependent patients, improving sleep and reducing anxiety.
Sleep Pressure Induction Study (1983)
Human | 25 nmol/kg IV | Single dose | 59% sleep increase
Subjects reported immediate sleep pressure sensation with 59% increase in total sleep time within 130 minutes, plus improved subsequent night sleep efficiency.
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